Preliminary safety assessment of a membrane-bound delta 9 desaturase candidate protein for transgenic oilseed crops.
نویسندگان
چکیده
A gene encoding delta 9 desaturase (D9DS), an integral membrane protein, is being considered for incorporation into oilseed crops to reduce saturated fatty acids and thus improve human nutritional value. Typically, a safety assessment for transgenic crops involves purifying heterologously produced transgenic proteins in an active form for use in safety studies. Membrane-bound proteins have been very difficult to isolate in an active form due to their inherent physicochemical properties. Described here are methods used to derive enriched preparations of the active D9DS protein for use in early stage safety studies. Results of these studies, in combination with bioinformatic results and knowledge of the mode of action of the protein, along with a history of safe consumption of related proteins, provides a weight of evidence supporting the safety of the D9DS protein in food and feed.
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ورودعنوان ژورنال:
- Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
دوره 50 10 شماره
صفحات -
تاریخ انتشار 2012